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KMID : 0379119870150030135
Korean Journal of Mycology
1987 Volume.15 No. 3 p.135 ~ p.141
Interspecific protoplast Fusion and Sexuality in Pleurotus
¹Ú¿ëȯ/Park, Yong Hwan
Àå±Ç¿­/À¯¿µº¹/À¯Ã¢Çö/ÀÌ¿¬Èñ/Á¸Æó¹öµð/Chang, Kwon Yawl/Yoo, Young Bok/You, Chang Hyun/Lee, Yeon Hee/Peberdy, John F.
Abstract
Interspecific heterokaryons were obtained by polyethylene glycol induced fusion of protoplaste from auxotrophic mutants of P. ostreatus+P. cornucopiae, P. ostreatus+P. florida, P. ostreatus+P. sajor-caju, P. florida+P. cornucopiae, P. florida+P. sajor-caju and P. sajor-caju+P. cornucopias protoplasts.
The Fusion products of protoplasts were induced by MCM+benomyl, but segregation of sectors were not identified. Interspecific fusion products of protoplasts between incompatible strains did not form true clamp connections and did not produce fruit bodies. For induction of fertility, interspecific heterokaryons crossed with their parents by hyphal anastomosis.
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